BioPAX pathway converted from "Synthesis of active ubiquitin: roles of E1 and E2 enzymes" in the Reactome database.
Synthesis of active ubiquitin: roles of E1 and E2 enzymes
Synthesis of active ubiquitin: roles of E1 and E2 enzymes
Ubiquitin monomers are processed from larger precursors and then activated by formation of a thiol ester bond between ubiquitin and a cysteine residue of an E1 activating enzyme (UBA1 or UBA6, Jin et al. 2007). The ubiquitin is then transferred to the active site cysteine residue of an E2 conjugating enzyme (reviewed in van Wijk and Timmers 2010, Kleiger and Mayor 2014, Stewart et al. 2016). Precursor proteins containing multiple ubiquitin monomers (polyubiquitins) are produced from the UBB and UBC genes. Precursors containing a single ubiquitin fused to a ribosomal protein are produced from the UBA52 and RPS27A genes. The proteases OTULIN and USP5 are very active in polyubiquitin processing, whereas the proteases UCHL3, USP7, and USP9X cleave the ubiquitin-ribosomal protein precursors yielding ubiquitin monomers (Grou et al. 2015). Other enzymes may also process ubiquitin precursors. A resultant ubiquitin monomer is activated by adenylation of its C-terminal glycine followed by conjugation of the C-terminus to a cysteine residue of the E1 enzymes UBA1 or UBA6 via a thiol ester bond (Jin et al. 2007, inferred from rabbit homologues in Haas et al. 1982, Hershko et al. 1983). The ubiquitin is then transferred from the E1 enzyme to a cysteine residue of one of several E2 enzymes (reviewed in van Wijk and Timmers 2010, Stewart et al. 2016).
Authored: May, Bruce, 2016-04-02
Reviewed: Azevedo, Jorge E, 2016-08-11
Edited: May, Bruce, 2016-04-02
3.4.19.12
OTULIN,USP5 cleaves UBB yielding ubiquitin
OTULIN,USP5 cleaves UBB yielding ubiquitin
The UBB poly-protein contains 3 Ubiquitin monomers that are released by proteolysis. Either USP5 (Falquet et al. 1995) or OTULIN can perform the proteolyic cleavage reactions (Grou et al. 2015).
Authored: May, Bruce, 2016-01-14
Reviewed: Azevedo, Jorge E, 2016-08-11
Edited: May, Bruce, 2016-01-14
Reactome DB_ID: 8853516
1
cytosol
GO
0005829
UniProt:P0CG47 UBB
UBB
UBB
SUBUNIT Interacts with SKP1-KMD2A and SKP1-KMD2B complexes.MISCELLANEOUS Ubiquitin is encoded by 4 different genes. UBA52 and RPS27A genes code for a single copy of ubiquitin fused to the ribosomal proteins L40 and S27a, respectively. UBB and UBC genes code for a polyubiquitin precursor with exact head to tail repeats, the number of repeats differ between species and strains.MISCELLANEOUS The mRNA encoding variant UBB(+1) is produced by an unknown mechanism involving the deletion of a GT dinucleotide in the close proximity of a GAGAG motif (PubMed:9422699). This variant mRNA is found in normal brain, but the encoded protein accumulates only in brain neurofibrillary tangles and neuritic plaques in Alzheimer disease and other tauopathies, as well as polyglutaminopathies (PubMed:14597671). UBB(+1) variant cannot be used for polyubiquitination, is not effectively degraded by the proteasome when ubiquitinated and ubiquitinated UBB(+1) is refractory to disassembly by deubiquitinating enzymes (DUBs). In healthy brain, UBB(+1) C-terminus can be cleaved by UCHL3 (PubMed:21762696).MISCELLANEOUS For a better understanding, features related to ubiquitin are only indicated for the first chain.SIMILARITY Belongs to the ubiquitin family.
Reactome
//www.joaskin.com
Homo sapiens
NCBI分类法
9606
UniProt
P0CG47
Chain Coordinates
1
EQUAL
229
EQUAL
Reactome DB_ID: 939230
1
153
EQUAL
228
EQUAL
Reactome DB_ID: 939213
1
77
EQUAL
152
EQUAL
Reactome DB_ID: 939214
1
1
EQUAL
76
EQUAL
PHYSIOL-LEFT-TO-RIGHT
ACTIVATION
Converted from EntitySet in Reactome
Reactome DB_ID: 8853527
OTULIN,USP5 [cytosol]
Converted from EntitySet in Reactome. Each synonym is a name of a PhysicalEntity, and each XREF points to one PhysicalEntity
USP5 [cytosol]
OTULIN [cytosol]
UniProt
P45974
UniProt
Q96BN8
GO
0004843
GO molecular function
Reactome Database ID Release 77
8853512
数据库标识符。使用这个URL连接到the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser?DB=gk_current&ID=8853512
Reactome Database ID Release 77
8853529
数据库标识符。使用这个URL连接到the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser?DB=gk_current&ID=8853529
Reactome
R-HSA-8853529
2
Reactome稳定的标识符。使用这个URL连接到the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser_st_id?ST_ID=R-HSA-8853529.2
26235645
Pubmed
2015
泛素的从头合成:识别of deubiquitinases acting on ubiquitin precursors
Grou, Cláudia P
Pinto, Manuel P
Mendes, Andreia V
Domingues, Pedro
Azevedo, Jorge E
Sci Rep 5:12836
7851534
Pubmed
1995
A human de-ubiquitinating enzyme with both isopeptidase and peptidase activities in vitro
Falquet, L
Paquet, N
Frutiger, S
Hughes, G J
Hoang-Van, K
Jaton, J C
FEBS Lett. 359:73-7
3.4.19.12
OTULIN,USP5 cleaves UBC yielding ubiquitin
OTULIN,USP5 cleaves UBC yielding ubiquitin
The UBC poly-protein contains 9 Ubiquitin monomers that are released by proteolysis. Either USP5 (Falquet et al. 1995) or OTULIN can perform the proteolyic cleavage reactions (Grou et al. 2015).
Authored: May, Bruce, 2016-01-14
Reviewed: Azevedo, Jorge E, 2016-08-11
Edited: May, Bruce, 2016-01-14
Reactome DB_ID: 8853531
1
UniProt:P0CG48 UBC
UBC
UBC
MISCELLANEOUS Ubiquitin is encoded by 4 different genes. UBA52 and RPS27A genes code for a single copy of ubiquitin fused to the ribosomal proteins L40 and S27a, respectively. UBB and UBC genes code for a polyubiquitin precursor with exact head to tail repeats, the number of repeats differ between species and strains.MISCELLANEOUS For the sake of clarity sequence features are annotated only for the first chain, and are not repeated for each of the following chains.SIMILARITY Belongs to the ubiquitin family.
UniProt
P0CG48
1
EQUAL
685
EQUAL
Reactome DB_ID: 939192
1
229
EQUAL
304
EQUAL
Reactome DB_ID: 939188
1
1
EQUAL
76
EQUAL
Reactome DB_ID: 939165
1
609
EQUAL
684
EQUAL
Reactome DB_ID: 939184
1
457
EQUAL
532
EQUAL
Reactome DB_ID: 939232
1
305
EQUAL
380
EQUAL
Reactome DB_ID: 939164
1
77
EQUAL
152
EQUAL
Reactome DB_ID: 939258
1
153
EQUAL
228
EQUAL
Reactome DB_ID: 939191
1
381
EQUAL
456
EQUAL
Reactome DB_ID: 939239
1
533
EQUAL
608
EQUAL
PHYSIOL-LEFT-TO-RIGHT
ACTIVATION
Converted from EntitySet in Reactome
Reactome DB_ID: 8853527
Reactome Database ID Release 77
8853515
数据库标识符。使用这个URL连接到the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser?DB=gk_current&ID=8853515
Reactome
R-HSA-8853515
2
Reactome稳定的标识符。使用这个URL连接到the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser_st_id?ST_ID=R-HSA-8853515.2
3.4.19.12
UCHL3,USP7,USP9X cleaves UBA52 yielding ubiquitin
UCHL3,USP7,USP9X cleaves UBA52 yielding ubiquitin
The UBA52 precursor protein contains the ribosomal protein L40 and ubiquitin (UBA52 residues 1 to 76) which are released by proteolysis. Any of the proteases UCHL3, USP7, or USP9X can catalyze the proteolysis reaction (Larsen et al. 1998, Grou et al. 2015).
Authored: May, Bruce, 2016-01-14
Reviewed: Azevedo, Jorge E, 2016-08-11
Edited: May, Bruce, 2016-01-14
Reactome DB_ID: 8853517
1
UniProt:P62987 UBA52
UBA52
UBCEP2
UBA52
SUBUNIT Ribosomal protein L40 is part of the 60S ribosomal subunit. Interacts with UBQLN1 (via UBA domain).MISCELLANEOUS Ubiquitin is encoded by 4 different genes. UBA52 and RPS27A genes code for a single copy of ubiquitin fused to the ribosomal proteins L40 and S27a, respectively. UBB and UBC genes code for a polyubiquitin precursor with exact head to tail repeats, the number of repeats differ between species and strains.MISCELLANEOUS For a better understanding, features related to ubiquitin are only indicated for the first chain.SIMILARITY In the N-terminal section; belongs to the ubiquitin family.SIMILARITY In the C-terminal section; belongs to the eukaryotic ribosomal protein eL40 family.
UniProt
P62987
1
EQUAL
128
EQUAL
Reactome DB_ID: 939203
1
1
EQUAL
76
EQUAL
Reactome DB_ID: 72476
1
77
EQUAL
128
EQUAL
PHYSIOL-LEFT-TO-RIGHT
ACTIVATION
Converted from EntitySet in Reactome
Reactome DB_ID: 8853523
UCHL3,USP7,USP9X [cytosol]
Converted from EntitySet in Reactome. Each synonym is a name of a PhysicalEntity, and each XREF points to one PhysicalEntity
USP9X [cytosol]
UCHL3 [cytosol]
USP7 [cytosol]
UniProt
Q93008
UniProt
P15374
UniProt
Q93009
Reactome Database ID Release 77
8853522
数据库标识符。使用这个URL连接到the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser?DB=gk_current&ID=8853522
Reactome Database ID Release 77
8853514
数据库标识符。使用这个URL连接到the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser?DB=gk_current&ID=8853514
Reactome
R-HSA-8853514
2
Reactome稳定的标识符。使用这个URL连接到the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser_st_id?ST_ID=R-HSA-8853514.2
9521656
Pubmed
1998
Substrate specificity of deubiquitinating enzymes: ubiquitin C-terminal hydrolases
Larsen, C N
Krantz, B A
Wilkinson, K D
Biochemistry 37:3358-68
3.4.19.12
UCHL3,USP7,USP9X cleaves RPS27A yielding ubiquitin
UCHL3,USP7,USP9X cleaves RPS27A yielding ubiquitin
The RPS27A precursor protein contains the ribosomal protein L40 and ubiquitin (RPS27A residues 1 to 76) which are released by proteolysis. Any of the proteases UCHL3, USP7, or USP9X can catalyze the proteolysis reaction (Larsen et al. 1998, Grou et al. 2015).
Authored: May, Bruce, 2016-01-14
Reviewed: Azevedo, Jorge E, 2016-08-11
Edited: May, Bruce, 2016-01-14
Reactome DB_ID: 8853524
1
UniProt:P62979 RPS27A
RPS27A
UBA80
RPS27A
UBCEP1
亚基核糖体蛋白S27a是40年代的一部分ribosomal subunit.MISCELLANEOUS Ubiquitin is encoded by 4 different genes. UBA52 and RPS27A genes code for a single copy of ubiquitin fused to the ribosomal proteins L40 and S27a, respectively. UBB and UBC genes code for a polyubiquitin precursor with exact head to tail repeats, the number of repeats differ between species and strains.MISCELLANEOUS For a better understanding, features related to ubiquitin are only indicated for the first chain.SIMILARITY In the N-terminal section; belongs to the ubiquitin family.SIMILARITY In the C-terminal section; belongs to the eukaryotic ribosomal protein eS31 family.
UniProt
P62979
1
EQUAL
76
EQUAL
Reactome DB_ID: 939205
1
1
EQUAL
76
EQUAL
Reactome DB_ID: 72363
1
77
EQUAL
156
EQUAL
PHYSIOL-LEFT-TO-RIGHT
ACTIVATION
Converted from EntitySet in Reactome
Reactome DB_ID: 8853523
Reactome Database ID Release 77
8853503
数据库标识符。使用这个URL连接到the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser?DB=gk_current&ID=8853503
Reactome
R-HSA-8853503
2
Reactome稳定的标识符。使用这个URL连接到the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser_st_id?ST_ID=R-HSA-8853503.2
6.2.1.45
UBA1 adenylates ubiquitin in the cytosol
UBA1 adenylates ubiquitin in the cytosol
Activation of ubiquitin by UBA1 proceeds through 3 steps: adenylation of ubiquitin, conjugation of ubiquitin from adenyl-ubiquitin to an internal cysteine residue of UBA1, and adenylation of a second molecule of ubiquitin. In the first step the adenylation module of UBA1 catalyzes the acyl-adenylation of the C-terminal glycine of ubiquitin (Jin et al. 2007, inferred from the rabbit homologue in Haas et al. 1982, Hershko et al. 1983, reviewed in Groettrup et al. 2008).
Authored: May, Bruce, 2016-01-11
Reviewed: Azevedo, Jorge E, 2016-08-11
Edited: May, Bruce, 2016-01-11
Reactome DB_ID: 947698
1
UniProt:P22314 UBA1
UBA1
A1S9T
UBA1
UBE1
FUNCTION Catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation through the ubiquitin-proteasome system (PubMed:1606621, PubMed:1447181). Activates ubiquitin by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding a ubiquitin-E1 thioester and free AMP (PubMed:1447181). Essential for the formation of radiation-induced foci, timely DNA repair and for response to replication stress. Promotes the recruitment of TP53BP1 and BRCA1 at DNA damage sites (PubMed:22456334).PATHWAY Protein modification; protein ubiquitination.SUBUNIT Monomer (By similarity). Interacts with GAN (via BTB domain).TISSUE SPECIFICITY Detected in erythrocytes (at protein level). Ubiquitous.DOMAIN The first 11 amino acids are essential for phosphorylation and exclusive nuclear localization.PTM ISGylated.MISCELLANEOUS There are two active sites within the E1 molecule, allowing it to accommodate two ubiquitin moieties at a time, with a new ubiquitin forming an adenylate intermediate as the previous one is transferred to the thiol site.SIMILARITY Belongs to the ubiquitin-activating E1 family.
UniProt
P22314
41
EQUAL
1058
EQUAL
Converted from EntitySet in Reactome
Reactome DB_ID: 113595
1
Ub [cytosol]
Converted from EntitySet in Reactome. Each synonym is a name of a PhysicalEntity, and each XREF points to one PhysicalEntity
UBC(229-304) [cytosol]
UBB(77-152) [cytosol]
UBA52(1-76) [cytosol]
UBC(1-76) [cytosol]
UBC(77-152) [cytosol]
UBC(381-456) [cytosol]
UBC(533-608) [cytosol]
UBB(153-228) [cytosol]
RPS27A(1-76) [cytosol]
UBB(1-76) [cytosol]
UBC(609-684) [cytosol]
UBC(457-532) [cytosol]
UBC(305-380) [cytosol]
UBC(153-228) [cytosol]
Reactome DB_ID: 113592
1
ATP(4-) [ChEBI:30616]
ATP(4-)
Adenosine 5'-triphosphate
atp
ATP
ChEBI
30616
Reactome DB_ID: 8852068
1
UBA1:泛素腺苷酸[cytosol]
UBA1:泛素腺苷酸
Converted from EntitySet in Reactome
Reactome DB_ID: 8852080
1
ubiquitin adenylate [cytosol]
Converted from EntitySet in Reactome. Each synonym is a name of a PhysicalEntity, and each XREF points to one PhysicalEntity
adenylatedG684-UBC(609-684) [cytosol]
adenylatedG304-UBC(229-304) [cytosol]
adenylatedG152-UBC(77-152) [cytosol]
adenylatedG608-UBC(533-608) [cytosol]
adenylatedG76-UBC(1-76) [cytosol]
adenylatedG228-UBC(153-228) [cytosol]
adenylatedG152-UBB(77-152) [cytosol]
adenylatedG76-UBA52(1-76) [cytosol]
adenylatedlG76-RPS27A(1-76) [cytosol]
adenylatedG456-UBC(381-456) [cytosol]
adenylatedG76-UBB(1-76) [cytosol]
adenylatedG532-UBC(457-532) [cytosol]
adenylatedG380-UBC(305-380) [cytosol]
adenylatedG228-UBB(153-228) [cytosol]
Reactome DB_ID: 947698
1
41
EQUAL
1058
EQUAL
Reactome Database ID Release 77
8852068
数据库标识符。使用这个URL连接到the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser?DB=gk_current&ID=8852068
Reactome
R-HSA-8852068
1
Reactome稳定的标识符。使用这个URL连接到the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser_st_id?ST_ID=R-HSA-8852068.1
Reactome DB_ID: 111294
1
diphosphoric acid [ChEBI:29888]
diphosphoric acid
ChEBI
29888
PHYSIOL-LEFT-TO-RIGHT
ACTIVATION
Reactome DB_ID: 947698
41
EQUAL
1058
EQUAL
GO
0004839
GO molecular function
Reactome Database ID Release 77
8865063
数据库标识符。使用这个URL连接到the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser?DB=gk_current&ID=8865063
Reactome Database ID Release 77
8852134
数据库标识符。使用这个URL连接到the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser?DB=gk_current&ID=8852134
Reactome
R-HSA-8852134
1
Reactome稳定的标识符。使用这个URL连接到the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser_st_id?ST_ID=R-HSA-8852134.1
17597759
Pubmed
2007
Dual E1 activation systems for ubiquitin differentially regulate E2 enzyme charging
Jin, J
Li, Xue
Gygi, SP
Harper, J Wade
Nature 447:1135-8
18353650
Pubmed
2008
Activating the ubiquitin family: UBA6 challenges the field
Groettrup, Marcus
Pelzer, Christiane
Schmidtke, Gunter
Hofmann, Kay
Trends Biochem. Sci. 33:230-7
6305978
Pubmed
1983
Components of ubiquitin-protein ligase system. Resolution, affinity purification, and role in protein breakdown
Hershko, A
Heller, H
Elias, S
Ciechanover, A
J. Biol. Chem. 258:8206-14
6277905
Pubmed
1982
Ubiquitin-activating enzyme. Mechanism and role in protein-ubiquitin conjugation
Haas, A L
Warms, J V
Hershko, A
Rose, I A
J. Biol. Chem. 257:2543-8
6.2.1.45
UBA1 conjugates ubiquitin to UBA1 in the cytosol
UBA1 conjugates ubiquitin to UBA1 in the cytosol
Activation of ubiquitin by UBA1 proceeds through 3 steps: adenylation of ubiquitin, conjugation of ubiquitin from adenyl-ubiquitin to an internal cysteine residue of UBA1, and adenylation of a second molecule of ubiquitin. In the second step, the thiol group of an internal cysteine residue of UBA1 attacks the acyl-adenyl bond of the C-terminal glycine of adenylated ubiquitin, resulting in a thioester bond between the cysteine residue and the C-terminal glycine of ubiquitin (Jin et al. 2007, inferred from the rabbit homologue in Haas et al. 1982, Hershko et al. 1983, reviewed in Groettrup et al. 2008).
Authored: May, Bruce, 2016-01-11
Reviewed: Azevedo, Jorge E, 2016-08-11
Edited: May, Bruce, 2016-01-11
Reactome DB_ID: 8852068
1
Reactome DB_ID: 8852063
1
Ub-Cys632-UBA1 [cytosol]
Ub-Cys632-UBA1
Reactome DB_ID: 947698
1
41
EQUAL
1058
EQUAL
Converted from EntitySet in Reactome
Reactome DB_ID: 113595
1
Reactome Database ID Release 77
8852063
数据库标识符。使用这个URL连接到the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser?DB=gk_current&ID=8852063
Reactome
R-HSA-8852063
1
Reactome稳定的标识符。使用这个URL连接到the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser_st_id?ST_ID=R-HSA-8852063.1
Reactome DB_ID: 76577
1
adenosine 5'-monophosphate [ChEBI:16027]
adenosine 5'-monophosphate
ChEBI
16027
PHYSIOL-LEFT-TO-RIGHT
ACTIVATION
Reactome DB_ID: 8852068
Reactome Database ID Release 77
8865083
数据库标识符。使用这个URL连接到the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser?DB=gk_current&ID=8865083
Reactome Database ID Release 77
8852133
数据库标识符。使用这个URL连接到the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser?DB=gk_current&ID=8852133
Reactome
R-HSA-8852133
1
Reactome稳定的标识符。使用这个URL连接到the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser_st_id?ST_ID=R-HSA-8852133.1
17580310
Pubmed
2007
UBE1L2, a novel E1 enzyme specific for ubiquitin
Pelzer, Christiane
Kassner, Ingrid
Matentzoglu, Konstantin
Singh, Rajesh K
Wollscheid, Hans-Peter
Scheffner, Martin
Schmidtke, Gunter
Groettrup, Marcus
J. Biol. Chem. 282:23010-4
6.2.1.45
Ub-Cys632-UBA1 adenylates ubiquitin in the cytosol
Ub-Cys632-UBA1 adenylates ubiquitin in the cytosol
Activation of ubiquitin by UBA1 proceeds through 3 steps: adenylation of ubiquitin, conjugation of ubiquitin from adenyl-ubiquitin to an internal cysteine residue of UBA1, and adenylation of a second molecule of ubiquitin. In the third step, the UBA1-ubiquitin conjugate adenylates the C-terminal glycine of a second ubiquitin molecule. This results in UBA1 loaded with 2 ubiquitin molecules, one of which is conjugated to an internal cysteine residue of UBA1 and one of which is adenylated and non-covalently bound to UBA1 (Jin et al. 2007, inferred from the rabbit homologue in Haas et al. 1982, Hershko et al. 1983, reviewed in Groettrup et al. 2008).
Authored: May, Bruce, 2016-01-11
Reviewed: Azevedo, Jorge E, 2016-08-11
Edited: May, Bruce, 2016-01-11
Reactome DB_ID: 8852063
1
Converted from EntitySet in Reactome
Reactome DB_ID: 113595
1
Reactome DB_ID: 113592
1
Reactome DB_ID: 8852079
1
Ub-Cys632-UBA1:泛素亚丁湾ylate [cytosol]
Ub-Cys632-UBA1:泛素亚丁湾ylate
Converted from EntitySet in Reactome
Reactome DB_ID: 8852080
1
Reactome DB_ID: 8852063
1
Reactome Database ID Release 77
8852079
数据库标识符。使用这个URL连接到the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser?DB=gk_current&ID=8852079
Reactome
R-HSA-8852079
1
Reactome稳定的标识符。使用这个URL连接到the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser_st_id?ST_ID=R-HSA-8852079.1
Reactome DB_ID: 111294
1
PHYSIOL-LEFT-TO-RIGHT
ACTIVATION
Reactome DB_ID: 8852063
Reactome Database ID Release 77
8865054
数据库标识符。使用这个URL连接到the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser?DB=gk_current&ID=8865054
Reactome Database ID Release 77
8852132
数据库标识符。使用这个URL连接到the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser?DB=gk_current&ID=8852132
Reactome
R-HSA-8852132
1
Reactome稳定的标识符。使用这个URL连接到the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser_st_id?ST_ID=R-HSA-8852132.1
UBA1 conjugates ubiquitin to cytosolic E2 enzymes
UBA1 conjugates ubiquitin to cytosolic E2 enzymes
In the cytosol, the UBA1-ubiquitin thiol ester conjugate transfers ubiquitin from UBA1 to an internal cysteine residue of the E2 enzyme, forming a thiol ester conjugate between ubiquitin and the cysteine residue of E2 (Jin et al. 2007, inferred from the rabbit homologue in Hershko et al. 1983). The E2 then disengages from UBA1 (Eletr et al. 2005).
Authored: May, Bruce, 2016-01-11
Reviewed: Azevedo, Jorge E, 2016-08-11
Edited: May, Bruce, 2016-01-11
Reactome DB_ID: 8852079
1
Converted from EntitySet in Reactome
Reactome DB_ID: 8852103
1
E2 ubiquitin conjugating enzyme (UBA1 substrate) [cytosol]
Converted from EntitySet in Reactome. Each synonym is a name of a PhysicalEntity, and each XREF points to one PhysicalEntity
UBE2S [cytosol]
UBE2D1(胞质)
UBE2Q2 [cytosol]
UBE2K [cytosol]
UBE2R2 [cytosol]
UBE2L3 [cytosol]
UBE2G2 [cytosol]
UBE2E3 [cytosol]
UBE2G1 [cytosol]
UBE2C [cytosol]
UBE2D2 [cytosol]
CDC34 [cytosol]
UBE2H [cytosol]
UniProt
Q16763
UniProt
P51668
UniProt
Q8WVN8
UniProt
P61086
UniProt
Q712K3
UniProt
P68036
UniProt
P60604
UniProt
Q969T4
UniProt
P62253
UniProt
O00762
UniProt
P62837
UniProt
P49427
UniProt
P62256
Reactome DB_ID: 8852251
1
Ub:E2 (UBA1 substrate) [cytosol]
Ub:E2 (UBA1 substrate)
Converted from EntitySet in Reactome
Reactome DB_ID: 8864150
1
Ub-conjugated E2 enzyme (UBA1 substrate) [cytosol]
Converted from EntitySet in Reactome. Each synonym is a name of a PhysicalEntity, and each XREF points to one PhysicalEntity
Ub-C92-UBE2K [cytosol]
Ub-C86-UBE2L3 [cytosol]
Ub-C87-UBE2H [cytosol]
Ub-C93-CDC34 [cytosol]
Ub-C85-UBE2D2 [cytosol]
Ub-C114-UBE2C [cytosol]
Ub-C90-UBE2G1 [cytosol]
Ub-C93-UBE2R2 [cytosol]
Ub-C89-UBE2G2 [cytosol]
Ub-C95-UBE2S [cytosol]
Ub-C304-UBE2Q2 [cytosol]
Ub-C145-UBE2E3 [cytosol]
Ub-C85-UBE2D1 [cytosol]
Converted from EntitySet in Reactome
Reactome DB_ID: 113595
1
Reactome Database ID Release 77
8852251
数据库标识符。使用这个URL连接到the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser?DB=gk_current&ID=8852251
Reactome
R-HSA-8852251
1
Reactome稳定的标识符。使用这个URL连接到the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser_st_id?ST_ID=R-HSA-8852251.1
Reactome DB_ID: 8852068
1
Reactome Database ID Release 77
8852129
数据库标识符。使用这个URL连接到the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser?DB=gk_current&ID=8852129
Reactome
R-HSA-8852129
1
Reactome稳定的标识符。使用这个URL连接到the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser_st_id?ST_ID=R-HSA-8852129.1
16142244
Pubmed
2005
E2 conjugating enzymes must disengage from their E1 enzymes before E3-dependent ubiquitin and ubiquitin-like transfer
Eletr, Ziad M
Huang, Danny T
Duda, David M
Schulman, Brenda A
Kuhlman, Brian
Nat. Struct. Mol. Biol. 12:933-4
6.2.1.45
UBA6 adenylates ubiquitin in the cytosol
UBA6 adenylates ubiquitin in the cytosol
从相应的UBA1推断,激活啊f ubiquitin by UBA6 (UBE1L1) proceeds through 3 steps: adenylation of ubiquitin, conjugation of ubiquitin from adenyl-ubiquitin to an internal cysteine residue of UBA6, and adenylation of a second molecule of ubiquitin. In the first step, the adenylation module of UBA6 catalyzes the acyl-adenylation of the C-terminal glycine of ubiquitin (Jin et al. 2007, Pelzer et al. 2007, Groettrup et al. 2008).
Authored: May, Bruce, 2016-01-11
Reviewed: Azevedo, Jorge E, 2016-08-11
Edited: May, Bruce, 2016-01-11
Converted from EntitySet in Reactome
Reactome DB_ID: 113595
1
Reactome DB_ID: 113592
1
Reactome DB_ID: 947701
1
UniProt:A0AVT1 UBA6
UBA6
UBE1L2
MOP4
UBA6
FUNCTION Activates ubiquitin by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding a ubiquitin-E1 thioester and free AMP. Specific for ubiquitin, does not activate ubiquitin-like peptides. Differs from UBE1 in its specificity for substrate E2 charging. Does not charge cell cycle E2s, such as CDC34. Essential for embryonic development. Required for UBD/FAT10 conjugation. Isoform 2 may play a key role in ubiquitin system and may influence spermatogenesis and male fertility.PATHWAY Protein modification; protein ubiquitination.SUBUNIT Forms a thioester with UBD in cells stimulated with tumor necrosis factor-alpha (TNFa) and interferon-gamma (IFNg) (PubMed:17889673, PubMed:25422469).TISSUE SPECIFICITY Widely expressed. Isoform 2 is predominantly expressed in testis with higher expression in adult testis than in fetal testis.SIMILARITY Belongs to the ubiquitin-activating E1 family.
UniProt
A0AVT1
1
EQUAL
1052
EQUAL
Reactome DB_ID: 8852088
1
UBA6:ubiquitin adenylate [cytosol]
UBA6:ubiquitin adenylate
Converted from EntitySet in Reactome
Reactome DB_ID: 8852080
1
Reactome DB_ID: 947701
1
1
EQUAL
1052
EQUAL
Reactome Database ID Release 77
8852088
数据库标识符。使用这个URL连接到the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser?DB=gk_current&ID=8852088
Reactome
R-HSA-8852088
1
Reactome稳定的标识符。使用这个URL连接到the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser_st_id?ST_ID=R-HSA-8852088.1
Reactome DB_ID: 111294
1
PHYSIOL-LEFT-TO-RIGHT
ACTIVATION
Reactome DB_ID: 947701
1
EQUAL
1052
EQUAL
Reactome Database ID Release 77
8865189
数据库标识符。使用这个URL连接到the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser?DB=gk_current&ID=8865189
Reactome Database ID Release 77
8865090
数据库标识符。使用这个URL连接到the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser?DB=gk_current&ID=8865090
Reactome
R-HSA-8865090
1
Reactome稳定的标识符。使用这个URL连接到the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser_st_id?ST_ID=R-HSA-8865090.1
6.2.1.45
UBA6 conjugates ubiquitin to UBA6 in the cytosol
UBA6 conjugates ubiquitin to UBA6 in the cytosol
从相应的UBA1推断,激活啊f ubiquitin by UBA6 (UBE1L1) proceeds through 3 steps: adenylation of ubiquitin, conjugation of ubiquitin from adenyl-ubiquitin to an internal cysteine residue of UBA6, and adenylation of a second molecule of ubiquitin. In the second step, the thiol group of an internal cysteine residue of UBA6 attacks the acyl-adenyl bond of the C-terminal glycine of adenylated ubiquitin, resulting in a thioester bond between the cysteine residue and the C-terminal glycine of ubiquitin (Jin et al. 2007, Pelzer et al. 2007, reviewed in Groettrup et al. 2008).
Authored: May, Bruce, 2016-01-11
Reviewed: Azevedo, Jorge E, 2016-08-11
Edited: May, Bruce, 2016-01-11
Reactome DB_ID: 8852088
1
Reactome DB_ID: 8852066
1
Ub-Cys625-UBA6 [cytosol]
Ub-Cys625-UBA6
Converted from EntitySet in Reactome
Reactome DB_ID: 113595
1
Reactome DB_ID: 947701
1
1
EQUAL
1052
EQUAL
Reactome Database ID Release 77
8852066
数据库标识符。使用这个URL连接到the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser?DB=gk_current&ID=8852066
Reactome
R-HSA-8852066
1
Reactome稳定的标识符。使用这个URL连接到the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser_st_id?ST_ID=R-HSA-8852066.1
Reactome DB_ID: 76577
1
PHYSIOL-LEFT-TO-RIGHT
ACTIVATION
Reactome DB_ID: 8852088
Reactome Database ID Release 77
8865076
数据库标识符。使用这个URL连接到the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser?DB=gk_current&ID=8865076
Reactome Database ID Release 77
8865098
数据库标识符。使用这个URL连接到the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser?DB=gk_current&ID=8865098
Reactome
R-HSA-8865098
2
Reactome稳定的标识符。使用这个URL连接到the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser_st_id?ST_ID=R-HSA-8865098.2
6.2.1.45
Ub-Cys625-UBA6 adenylates ubiquitin in the cytosol
Ub-Cys625-UBA6 adenylates ubiquitin in the cytosol
从相应的UBA1推断,激活啊f ubiquitin by UBA6 (UBE1L1) proceeds through 3 steps: adenylation of ubiquitin, conjugation of ubiquitin from adenyl-ubiquitin to an internal cysteine residue of UBA6, and adenylation of a second molecule of ubiquitin. In the third step, the UBA6-ubiquitin conjugate adenylates the C-terminal glycine of a second ubiquitin molecule. This results in UBA6 loaded with 2 ubiquitin molecules, one of which is conjugated to an internal cysteine residue of UBA6 and one of which is adenylated and non-covalently bound to UBA6 (Jin et al. 2007, Pelzer et al. 2007, reviewed in Groettrup et al. 2008).
Authored: May, Bruce, 2016-01-11
Reviewed: Azevedo, Jorge E, 2016-08-11
Edited: May, Bruce, 2016-01-11
Reactome DB_ID: 8852066
1
Converted from EntitySet in Reactome
Reactome DB_ID: 113595
1
Reactome DB_ID: 113592
1
Reactome DB_ID: 111294
1
Reactome DB_ID: 8852097
1
Ub-Cys625-UBA6:ubiquitin adenylate [cytosol]
Ub-Cys625-UBA6:ubiquitin adenylate
Converted from EntitySet in Reactome
Reactome DB_ID: 8852080
1
Reactome DB_ID: 8852066
1
Reactome Database ID Release 77
8852097
数据库标识符。使用这个URL连接到the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser?DB=gk_current&ID=8852097
Reactome
R-HSA-8852097
1
Reactome稳定的标识符。使用这个URL连接到the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser_st_id?ST_ID=R-HSA-8852097.1
PHYSIOL-LEFT-TO-RIGHT
ACTIVATION
Reactome DB_ID: 8852066
Reactome Database ID Release 77
8865193
数据库标识符。使用这个URL连接到the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser?DB=gk_current&ID=8865193
Reactome Database ID Release 77
8865050
数据库标识符。使用这个URL连接到the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser?DB=gk_current&ID=8865050
Reactome
R-HSA-8865050
1
Reactome稳定的标识符。使用这个URL连接到the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser_st_id?ST_ID=R-HSA-8865050.1
UBA6 conjugates ubiquitin to cytosolic E2 enzymes
UBA6 conjugates ubiquitin to cytosolic E2 enzymes
In the cytosol, the UBA6-ubiquitin thiol ester conjugate transfers ubiquitin from UBA6 to an internal cysteine residue of the E2 enzyme, forming a thiol ester conjugate between ubiquitin and the cysteine residue of E2 (Jin et al. 2007, inferred from the rabbit homologue in Hershko et al. 1983, reviewed in Groettrup et al. 2008). As inferred from UBA1, the E2 then disengages from UBA6 (Eletr et al. 2005).
Authored: May, Bruce, 2016-01-11
Reviewed: Azevedo, Jorge E, 2016-08-11
Edited: May, Bruce, 2016-01-11
Converted from EntitySet in Reactome
Reactome DB_ID: 8852061
1
E2 ubiquitin conjugating enzyme (UBA6 substrate) [cytosol]
Converted from EntitySet in Reactome. Each synonym is a name of a PhysicalEntity, and each XREF points to one PhysicalEntity
UBE2L3 [cytosol]
UBE2G2 [cytosol]
UBE2E3 [cytosol]
UBE2Z [cytosol]
UBE2S [cytosol]
UBE2D1(胞质)
UBE2D2 [cytosol]
UniProt
Q9H832
Reactome DB_ID: 8852097
1
Reactome DB_ID: 8864229
1
Ub:E2 (UBA6 substrate) [cytosol]
Ub:E2 (UBA6 substrate)
Converted from EntitySet in Reactome
Reactome DB_ID: 113595
1
Converted from EntitySet in Reactome
Reactome DB_ID: 8864192
1
Ub-conjugated E2 enzyme (UBA6 substrate) [cytosol]
Converted from EntitySet in Reactome. Each synonym is a name of a PhysicalEntity, and each XREF points to one PhysicalEntity
Ub-C188-UBE2Z [cytosol]
Ub-C89-UBE2G2 [cytosol]
Ub-C95-UBE2S [cytosol]
Ub-C86-UBE2L3 [cytosol]
Ub-C145-UBE2E3 [cytosol]
Ub-C85-UBE2D1 [cytosol]
Ub-C85-UBE2D2 [cytosol]
Reactome Database ID Release 77
8864229
数据库标识符。使用这个URL连接到the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser?DB=gk_current&ID=8864229
Reactome
R-HSA-8864229
1
Reactome稳定的标识符。使用这个URL连接到the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser_st_id?ST_ID=R-HSA-8864229.1
Reactome DB_ID: 8852088
1
Reactome Database ID Release 77
8852127
数据库标识符。使用这个URL连接到the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser?DB=gk_current&ID=8852127
Reactome
R-HSA-8852127
1
Reactome稳定的标识符。使用这个URL连接到the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser_st_id?ST_ID=R-HSA-8852127.1
UBA1 adenylates ubiquitin in the nucleoplasm
UBA1 adenylates ubiquitin in the nucleoplasm
UBA1 is present in both the cytosol and nucleoplasm (Grenfell et al. 1994). Activation of ubiquitin by UBA1 proceeds through 3 steps: adenylation of ubiquitin, conjugation of ubiquitin from adenyl-ubiquitin to an internal cysteine residue of UBA1, and adenylation of a second molecule of ubiquitin. In the first step, the adenylation module of UBA1 catalyzes the acyl-adenylation of the C-terminal glycine of ubiquitin (Jin et al. 2007, inferred from the rabbit homologue in Haas et al. 1982, Hershko et al. 1983, reviewed in Groettrup et al. 2008).
Authored: May, Bruce, 2016-01-11
Reviewed: Azevedo, Jorge E, 2016-08-11
Edited: May, Bruce, 2016-01-11
Reactome DB_ID: 8852116
1
nucleoplasm
GO
0005654
2
EQUAL
1058
EQUAL
Reactome DB_ID: 29358
1
Converted from EntitySet in Reactome
Reactome DB_ID: 68524
1
Ub [nucleoplasm]
Converted from EntitySet in Reactome. Each synonym is a name of a PhysicalEntity, and each XREF points to one PhysicalEntity
UBC(533-608) [nucleoplasm]
UBC(305-380) [nucleoplasm]
UBC(229-304) [nucleoplasm]
UBC(457-532) [nucleoplasm]
UBC(1-76) [nucleoplasm]
UBA52(1-76) [nucleoplasm]
UBC(153-228) [nucleoplasm]
UBB(1-76) [nucleoplasm]
UBC(381-456) [nucleoplasm]
UBC(609-684) [nucleoplasm]
UBB(77-152) [nucleoplasm]
RPS27A(1-76) [nucleoplasm]
UBB(153-228) [nucleoplasm]
UBC(77-152) [nucleoplasm]
Reactome DB_ID: 8852055
1
UBA1:泛素腺苷酸[nucleoplasm]
UBA1:泛素腺苷酸
Reactome DB_ID: 8852116
1
2
EQUAL
1058
EQUAL
Converted from EntitySet in Reactome
Reactome DB_ID: 8852106
1
ubiquitin adenylate [nucleoplasm]
Converted from EntitySet in Reactome. Each synonym is a name of a PhysicalEntity, and each XREF points to one PhysicalEntity
adenylatedG152-UBB(77-152) [nucleoplasm]
adenylatedG228-UBB(153-228) [nucleoplasm]
adenylatedG456-UBC(381-456) [nucleoplasm]
adenylatedG684-UBC(609-684) [nucleoplasm]
adenylatedG608-UBC(533-608) [nucleoplasm]
adenylatedG380-UBC(305-380) [nucleoplasm]
adenylatedG76-UBB(1-76) [nucleoplasm]
adenylatedlG76-RPS27A(1-76) [nucleoplasm]
adenylatedG76-UBC(1-76) [nucleoplasm]
adenylatedG304-UBC(229-304) [nucleoplasm]
adenylatedG228-UBC(153-228) [nucleoplasm]
adenylatedG152-UBC(77-152) [nucleoplasm]
adenylatedG532-UBC(457-532) [nucleoplasm]
adenylatedG76-UBA52(1-76) [nucleoplasm]
Reactome Database ID Release 77
8852055
数据库标识符。使用这个URL连接到the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser?DB=gk_current&ID=8852055
Reactome
R-HSA-8852055
1
Reactome稳定的标识符。使用这个URL连接到the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser_st_id?ST_ID=R-HSA-8852055.1
Reactome DB_ID: 113541
1
Reactome Database ID Release 77
8852128
数据库标识符。使用这个URL连接到the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser?DB=gk_current&ID=8852128
Reactome
R-HSA-8852128
2
Reactome稳定的标识符。使用这个URL连接到the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser_st_id?ST_ID=R-HSA-8852128.2
8010951
Pubmed
1994
Nuclear localization of the ubiquitin-activating enzyme, E1, is cell-cycle-dependent
Grenfell, S J
Trausch-Azar, J S
Handley-Gearhart, P M
Ciechanover, A
Schwartz, A L
Biochem. J. 300:701-8
UBA1 conjugates ubiquitin to UBA1 in the nucleus
UBA1 conjugates ubiquitin to UBA1 in the nucleus
UBA1 is located in both the nucleus and cytoplasm (Grenfell 1994). Activation of ubiquitin by UBA1 proceeds through 3 steps: adenylation of ubiquitin, conjugation of ubiquitin from adenyl-ubiquitin to an internal cysteine residue of UBA1, and adenylation of a second molecule of ubiquitin. In the second step, the thiol group of an internal cysteine residue of UBA1 attacks the acyl-adenyl bond of the C-terminal glycine of adenylated ubiquitin, resulting in a thioester bond between the cysteine residue and the C-terminal glycine of ubiquitin (Jin et al. 2007, inferred from the rabbit homologue in Haas et al. 1982, Hershko et al. 1983, reviewed in Groettrup et al. 2008).
Authored: May, Bruce, 2016-01-11
Reviewed: Azevedo, Jorge E, 2016-08-11
Edited: May, Bruce, 2016-01-11
Reactome DB_ID: 8852055
1
Reactome DB_ID: 164121
1
Reactome DB_ID: 8852095
1
Ub-Cys632-UBA1 [nucleoplasm]
Ub-Cys632-UBA1
Reactome DB_ID: 8852116
1
2
EQUAL
1058
EQUAL
Converted from EntitySet in Reactome
Reactome DB_ID: 68524
1
Reactome Database ID Release 77
8852095
数据库标识符。使用这个URL连接到the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser?DB=gk_current&ID=8852095
Reactome
R-HSA-8852095
2
Reactome稳定的标识符。使用这个URL连接到the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser_st_id?ST_ID=R-HSA-8852095.2
Reactome Database ID Release 77
8852136
数据库标识符。使用这个URL连接到the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser?DB=gk_current&ID=8852136
Reactome
R-HSA-8852136
1
Reactome稳定的标识符。使用这个URL连接到the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser_st_id?ST_ID=R-HSA-8852136.1
Ub-Cys632-UBA1 adenylates ubiquitin in the nucleus
Ub-Cys632-UBA1 adenylates ubiquitin in the nucleus
UBA1 is located in both the cytoplasm and nucleus (Grenfell et al. 1994). Activation of ubiquitin by UBA1 proceeds through 3 steps: adenylation of ubiquitin, conjugation of ubiquitin from adenyl-ubiquitin to an internal cysteine residue of UBA1, and adenylation of a second molecule of ubiquitin. In the third step, the UBA1-ubiquitin conjugate adenylates the C-terminal glycine of a second ubiquitin molecule. This results in UBA1 loaded with 2 ubiquitin molecules, one of which is conjugated to an internal cysteine residue of UBA1 and one of which is adenylated and non-covalently bound to UBA1 (Jin et al. 2007, inferred from the rabbit homologue in Haas et al. 1982, Hershko et al. 1983, reviewed in Groettrup et al. 2008)
Authored: May, Bruce, 2016-01-11
Reviewed: Azevedo, Jorge E, 2016-08-11
Edited: May, Bruce, 2016-01-11
Reactome DB_ID: 8852095
1
Reactome DB_ID: 29358
1
Converted from EntitySet in Reactome
Reactome DB_ID: 68524
1
Reactome DB_ID: 8852059
1
Ub-Cys632-UBA1:泛素亚丁湾ylate [nucleoplasm]
Ub-Cys632-UBA1:泛素亚丁湾ylate
Converted from EntitySet in Reactome
Reactome DB_ID: 8852106
1
Reactome DB_ID: 8852095
1
Reactome Database ID Release 77
8852059
数据库标识符。使用这个URL连接到the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser?DB=gk_current&ID=8852059
Reactome
R-HSA-8852059
1
Reactome稳定的标识符。使用这个URL连接到the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser_st_id?ST_ID=R-HSA-8852059.1
Reactome DB_ID: 113541
1
Reactome Database ID Release 77
8852131
数据库标识符。使用这个URL连接到the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser?DB=gk_current&ID=8852131
Reactome
R-HSA-8852131
2
Reactome稳定的标识符。使用这个URL连接到the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser_st_id?ST_ID=R-HSA-8852131.2
UBA1 conjugates ubiquitin to nuclear E2 enzymes
UBA1 conjugates ubiquitin to nuclear E2 enzymes
In the nucleus (Grenfell et al. 1994), the UBA1-ubiquitin thiol ester conjugate transfers ubiquitin from UBA1 to an internal cysteine residue of the E2 enzyme, forming a thiol ester conjugate between ubiquitin and the cysteine residue of E2 (Jin et al. 2007, inferred from the rabbit homologue in Hershko et al. 1983, reviewed in Groettrup et al. 2008). The E2 then disengages from UBA1 (Eletr et al. 2005).
Authored: May, Bruce, 2016-01-11
Reviewed: Azevedo, Jorge E, 2016-08-11
Edited: May, Bruce, 2016-01-11
Reactome DB_ID: 8852059
1
Converted from EntitySet in Reactome
Reactome DB_ID: 8852109
1
E2 ubiquitin conjugating enzyme (UBA1 substrate) [nucleoplasm]
Converted from EntitySet in Reactome. Each synonym is a name of a PhysicalEntity, and each XREF points to one PhysicalEntity
CDC34 [nucleoplasm]
UBE2E3 [nucleoplasm]
UBE2B [nucleoplasm]
UBE2L3 [nucleoplasm]
UBE2E1 [nucleoplasm]
UBE2D2 [nucleoplasm]
UBE2S [nucleoplasm]
UBE2A [nucleoplasm]
UBE2T(νcleoplasm]
UBE2C [nucleoplasm]
UBE2W [nucleoplasm]
UniProt
P63146
UniProt
P51965
UniProt
P49459
UniProt
Q9NPD8
UniProt
Q96B02
Reactome DB_ID: 8852055
1
Reactome DB_ID: 8864169
1
Ub:E2 (UBA1 substrate) [nucleoplasm]
Ub:E2 (UBA1 substrate)
Converted from EntitySet in Reactome
Reactome DB_ID: 8864197
1
Ub-conjugated E2 enzyme (UBA1 substrate) [nucleoplasm]
Converted from EntitySet in Reactome. Each synonym is a name of a PhysicalEntity, and each XREF points to one PhysicalEntity
Ub-C91-UBE2W [nucleoplasm]
Ub-C88-UBE2B [nucleoplasm]
Ub-C131-UBE2E1 [nucleoplasm]
Ub-C145-UBE2E3 [nucleoplasm]
Ub-C86-UBE2L3 [nucleoplasm]
Ub-C95-UBE2S [nucleoplasm]
Ub-C88-UBE2A [nucleoplasm]
Ub-C86-UBE2T [nucleoplasm]
Ub-C85-UBE2D2 [nucleoplasm]
Ub-C114-UBE2C [nucleoplasm]
Ub-C93-CDC34 [nucleoplasm]
Converted from EntitySet in Reactome
Reactome DB_ID: 68524
1
Reactome Database ID Release 77
8864169
数据库标识符。使用这个URL连接到the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser?DB=gk_current&ID=8864169
Reactome
R-HSA-8864169
2
Reactome稳定的标识符。使用这个URL连接到the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser_st_id?ST_ID=R-HSA-8864169.2
Reactome Database ID Release 77
8852130
数据库标识符。使用这个URL连接到the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser?DB=gk_current&ID=8852130
Reactome
R-HSA-8852130
1
Reactome稳定的标识符。使用这个URL连接到the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser_st_id?ST_ID=R-HSA-8852130.1
Reactome Database ID Release 77
8866652
数据库标识符。使用这个URL连接到the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser?DB=gk_current&ID=8866652
Reactome
R-HSA-8866652
2
Reactome稳定的标识符。使用这个URL连接到the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser_st_id?ST_ID=R-HSA-8866652.2
24457024
Pubmed
2014
Perilous journey: a tour of the ubiquitin-proteasome system
Kleiger, Gary
Mayor, Thibault
Trends Cell Biol. 24:352-9
19940261
Pubmed
2010
The family of ubiquitin-conjugating enzymes (E2s): deciding between life and death of proteins
van Wijk, SJ
timmer, HT
FASEB J 24:981-93
27002219
Pubmed
2016
E2 enzymes: more than just middle men
Stewart, Mikaela D
Ritterhoff, Tobias
Klevit, Rachel E
Brzovic, Peter S
Cell Res. 26:423-40
GO
0016567
GO biological process