The inference is based on the homology mapping from PANTHER. Briefly, reactions for which all involved PhysicalEntities (in input, output and catalyst) have a mapped orthologue/paralogue (for complexes at least 75% of components must have a mapping) are inferred to the other species. High level events are also inferred for these events to allow for easier navigation.

More details and caveats of the event inference in Reactome. For details on PANTHER see also: http://www.pantherdb.org/about.jsp RB1 binds condensin II RB1 binds condensin II This event has been computationally inferred from an event that has been demonstrated in another species.

The inference is based on the homology mapping from PANTHER. Briefly, reactions for which all involved PhysicalEntities (in input, output and catalyst) have a mapped orthologue/paralogue (for complexes at least 75% of components must have a mapping) are inferred to the other species. High level events are also inferred for these events to allow for easier navigation.

More details and caveats of the event inference in Reactome. For details on PANTHER see also: http://www.pantherdb.org/about.jsp Converted from EntitySet in Reactome Reactome DB_ID: 10497102 1 nucleoplasm GO 0005654 Homologues of RB1 [nucleoplasm] Converted from EntitySet in Reactome. Each synonym is a name of a PhysicalEntity, and each XREF points to one PhysicalEntity Rbf [nucleoplasm] Rbf2 [nucleoplasm] Reactome //www.joaskin.com Drosophila melanogaster NCBI Taxonomy 7227 UniProt Q24472 UniProt Q9VF04 Reactome DB_ID: 10535958 1 Condensin II [nucleoplasm] Condensin II Reactome DB_ID: 10535956 1 UniProt:Q8INL2 Cap-H2 UniProt Q8INL2 Chain Coordinates 1 EQUAL 605 EQUAL Reactome DB_ID: 10535951 1 Ghost homologue of NCAPG2 [nucleoplasm] Ghost homologue of NCAPG2 Reactome DB_ID: 10535949 1 UniProt:Q9VMQ4 Cap-D3 UniProt Q9VMQ4 1 EQUAL 1498 EQUAL Reactome DB_ID: 10535944 1 UniProt:Q9V3A7 glu UniProt Q9V3A7 1 EQUAL 1288 EQUAL Reactome DB_ID: 10535939 1 UniProt:Q7KK96 SMC2 UniProt Q7KK96 1 EQUAL 1197 EQUAL Reactome Database ID Release 77 10535958 数据库标识符。使用这个URL连接到the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser?DB=gk_current&ID=10535958 Reactome R-DME-1638144 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser_st_id?ST_ID=R-DME-1638144.1 Reactome DB_ID: 10535960 1 Condensin II:RB1 [nucleoplasm] Condensin II:RB1 Converted from EntitySet in Reactome Reactome DB_ID: 10497102 1 Reactome DB_ID: 10535958 1 Reactome Database ID Release 77 10535960 数据库标识符。使用这个URL连接到the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser?DB=gk_current&ID=10535960 Reactome R-DME-2172665 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser_st_id?ST_ID=R-DME-2172665.1 Reactome Database ID Release 77 10535962 数据库标识符。使用这个URL连接到the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser?DB=gk_current&ID=10535962 Reactome R-DME-2172666 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser_st_id?ST_ID=R-DME-2172666.1 通过interac RB1结合condensin II复杂tion with the NCAPD3 subunit of condensin II. This interaction is E2F independent and is important for targeting of the condensin II complex to chromatin (Longworth et al. 2008). RB1 may be particularly important for targeting of the condensin II complex to centromeres (Manning et al. 2010). RB1 deficient cells exhibit chromosome condensation defects and are prone to aneuploidy caused by aberrant chromosomal segregation. Therefore, tumor suppressor role of RB1 is based both on E2F-dependent control of G1/S transition, as well as on E2F-independent maintenance of genomic stability through regulation of mitotic chromosome condensation (Longworth et al. 20008, Coschi et al. 2010, Manning et al. 2010).

The role of RB1 in the maintenance of genomic stability is supported by studies of the childhood eye cancer retinoblastoma and its precursor, retinoma. Retinoma, a quiescent precursor of malignant retinoblastoma with functional loss of both RB1 alleles, is genomically unstable (Dimaras et al. 2008). Also, while the majority of retinoblastoma tumors are caused by the loss-of-function of the tumor suppressor gene RB1, ~2% of retinoblastoma tumors in unilaterally affected patients are initiated by a high level amplification of MYCN gene, in the presence of two functional, unmutated RB1 alleles. These tumors, with normal RB1 and amplified MYCN show a much lower level of genomic instability than retinoblastoma tumors with RB1 loss-of-function (Rushlow et al. 2013). 18367646 Pubmed 2008 RBF1 promotes chromatin condensation through a conserved interaction with the Condensin II protein dCAP-D3 Longworth, Michelle Herr, A Ji, JY Dyson, NJ Genes Dev 22:1011-24 20551165 Pubmed 2010 Loss of pRB causes centromere dysfunction and chromosomal instability Manning, AL Longworth, Michelle Dyson, NJ Genes Dev 24:1364-76 18211953 Pubmed 2008 Loss of RB1 induces non-proliferative retinoma: increasing genomic instability correlates with progression to retinoblastoma Dimaras, Helen Khetan, Vikas Halliday, William Orlic, Marija Prigoda, Nadia L Piovesan, Beata Marrano, Paula Corson, Timothy W Eagle, Ralph C Squire, Jeremy A Gallie, Brenda L Hum. Mol. Genet. 17:1363-72 20551166 Pubmed 2010 Mitotic chromosome condensation mediated by the retinoblastoma protein is tumor-suppressive Coschi, Courtney H Martens, Alison L Ritchie, Kieran Francis, Sarah M Chakrabarti, Subrata Berube, Nathalie G Dick, Frederick Andrew Genes Dev. 24:1351-63 23498719 Pubmed 2013 Characterisation of retinoblastomas without RB1 mutations: genomic, gene expression, and clinical studies Rushlow, Diane E Mol, Berber M Kennett, Jennifer Y Yee, Stephanie Pajovic, Sanja Thériault, Brigitte L Prigoda-Lee, Nadia L Spencer, Clarellen Dimaras, Helen Corson, Timothy W Pang, Renée Massey, Christine Godbout, Roseline Jiang, Zhe Zacksenhaus, Eldad Paton, Katherine Moll, Annette C Houdayer, Claude Raizis, Anthony Halliday, William Lam, Wan L Boutros, Paul C 罗曼,说是 Dorsman, Josephine C Gallie, Brenda L Lancet Oncol. 14:327-34 inferred by electronic annotation IEA GO IEA 2.7.11.22 CDK1 phosphorylates condensin II subunit NCAPD3 CDK1 phosphorylates condensin II subunit NCAPD3 This event has been computationally inferred from an event that has been demonstrated in another species.

The inference is based on the homology mapping from PANTHER. Briefly, reactions for which all involved PhysicalEntities (in input, output and catalyst) have a mapped orthologue/paralogue (for complexes at least 75% of components must have a mapping) are inferred to the other species. High level events are also inferred for these events to allow for easier navigation.

More details and caveats of the event inference in Reactome. For details on PANTHER see also: http://www.pantherdb.org/about.jsp Reactome DB_ID: 10536227 1 Condensin II:Nucleosome with H4K20me1 [nucleoplasm] Condensin II:Nucleosome with H4K20me1 Reactome DB_ID: 10536199 1 Nucleosome with H3K4me2/3:H4K20me1 [nucleoplasm] Nucleosome with H3K4me2/3:H4K20me1 Reactome DB_ID: 10536197 2 Ghost homologue of MeK-HIST1H4A [nucleoplasm] Ghost homologue of MeK-HIST1H4A Reactome DB_ID: 10536195 2 Ghost homologue of H3K4me2/3 [nucleoplasm] Ghost homologue of H3K4me2/3 Converted from EntitySet in Reactome Reactome DB_ID: 10501529 2 Histone H2A [nucleoplasm] Converted from EntitySet in Reactome. Each synonym is a name of a PhysicalEntity, and each XREF points to one PhysicalEntity Converted from EntitySet in Reactome Reactome DB_ID: 10501538 2 Histone H2B [nucleoplasm] Converted from EntitySet in Reactome. Each synonym is a name of a PhysicalEntity, and each XREF points to one PhysicalEntity Reactome Database ID Release 77 10536199 数据库标识符。使用这个URL连接到the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser?DB=gk_current&ID=10536199 Reactome R-DME-2245190 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser_st_id?ST_ID=R-DME-2245190.1 Reactome DB_ID: 10535958 1 Reactome Database ID Release 77 10536227 数据库标识符。使用这个URL连接到the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser?DB=gk_current&ID=10536227 Reactome R-DME-2288093 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser_st_id?ST_ID=R-DME-2288093.1 Reactome DB_ID: 29358 1 ATP(4-) [ChEBI:30616] ATP(4-) Adenosine 5'-triphosphate atp ATP ChEBI 30616 Reactome DB_ID: 10536223 1 Phosphorylated Condensin II:Nucleosome [nucleoplasm] Phosphorylated Condensin II:Nucleosome Reactome DB_ID: 10536199 1 Reactome DB_ID: 10536204 1 Phosphorylated Condensin II [nucleoplasm] Phosphorylated Condensin II Reactome DB_ID: 10535956 1 1 EQUAL 605 EQUAL Reactome DB_ID: 10536202 1 O-phospho-L-threonine at 1415 (in Homo sapiens) 1415 EQUAL O-phospho-L-threonine [MOD:00047] 1 EQUAL 1498 EQUAL Reactome DB_ID: 10535951 1 Reactome DB_ID: 10535944 1 1 EQUAL 1288 EQUAL Reactome DB_ID: 10535939 1 1 EQUAL 1197 EQUAL Reactome Database ID Release 77 10536204 数据库标识符。使用这个URL连接到the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser?DB=gk_current&ID=10536204 Reactome R-DME-2294584 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser_st_id?ST_ID=R-DME-2294584.1 Reactome Database ID Release 77 10536223 数据库标识符。使用这个URL连接到the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser?DB=gk_current&ID=10536223 Reactome R-DME-2294607 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser_st_id?ST_ID=R-DME-2294607.1 Reactome DB_ID: 113582 1 ADP(3-) [ChEBI:456216] ADP(3-) ADP trianion 5'-O-[(phosphonatooxy)phosphinato]adenosine ADP ChEBI 456216 PHYSIOL-LEFT-TO-RIGHT ACTIVATION Reactome DB_ID: 10507171 CCNB1:p-T161-CDK1 [nucleoplasm] CCNB1:p-T161-CDK1 Reactome DB_ID: 10507080 1 UniProt:P23572 Cdk1 UniProt P23572 O-phospho-L-threonine at 161 (in Homo sapiens) 161 EQUAL 1 EQUAL 297 EQUAL Reactome DB_ID: 10507068 1 UniProt:P20439 CycB UniProt P20439 1 EQUAL 433 EQUAL Reactome Database ID Release 77 10507171 数据库标识符。使用这个URL连接到the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser?DB=gk_current&ID=10507171 Reactome R-DME-170160 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser_st_id?ST_ID=R-DME-170160.1 GO 0004693 GO molecular function Reactome Database ID Release 77 10507775 数据库标识符。使用这个URL连接到the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser?DB=gk_current&ID=10507775 Reactome Database ID Release 77 10536229 数据库标识符。使用这个URL连接到the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser?DB=gk_current&ID=10536229 Reactome R-DME-2294600 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser_st_id?ST_ID=R-DME-2294600.1 Phosphorylation of the threonine residue T1415 of condensin II subunit NCAPD3 is required for chromosome condensation in prophase. In vivo, phosphorylation of NCAPD3 threonine residue T1415 is blocked when cells are treated with CDK1 inhibitors. In addition, it was shown that CDK1 in complex with cyclin B1 (CDK1:CCNB1) phosphorylates NCAPD3 at T1415 in vitro (Abe et al. 2011). 21498573 Pubmed 2011 The initial phase of chromosome condensation requires Cdk1-mediated phosphorylation of the CAP-D3 subunit of condensin II Abe, Satoshi Nagasaka, Kota Hirayama, Youko Kozuka-Hata, H Oyama, M Aoyagi, Yutaka Obuse, Chikashi Hirota, Toru Genes Dev. 25:863-74 inferred by electronic annotation IEA GO IEA PLK1 binds phosphorylated condensin II complex PLK1 binds phosphorylated condensin II complex This event has been computationally inferred from an event that has been demonstrated in another species.

The inference is based on the homology mapping from PANTHER. Briefly, reactions for which all involved PhysicalEntities (in input, output and catalyst) have a mapped orthologue/paralogue (for complexes at least 75% of components must have a mapping) are inferred to the other species. High level events are also inferred for these events to allow for easier navigation.

More details and caveats of the event inference in Reactome. For details on PANTHER see also: http://www.pantherdb.org/about.jsp Reactome DB_ID: 10536223 1 Reactome DB_ID: 10504772 1 UniProt:P52304 polo UniProt P52304 O-phospho-L-threonine at 210 (in Homo sapiens) 210 EQUAL 1 EQUAL 603 EQUAL Reactome DB_ID: 10536206 1 p-T210-PLK1:Phosphorylated Condensin II:Nucleosome [nucleoplasm] p-T210-PLK1:Phosphorylated Condensin II:Nucleosome Reactome DB_ID: 10536199 1 Reactome DB_ID: 10536204 1 Reactome DB_ID: 10504772 1 O-phospho-L-threonine at 210 (in Homo sapiens) 210 EQUAL 1 EQUAL 603 EQUAL Reactome Database ID Release 77 10536206 数据库标识符。使用这个URL连接到the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser?DB=gk_current&ID=10536206 Reactome R-DME-2294591 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser_st_id?ST_ID=R-DME-2294591.1 Reactome Database ID Release 77 10536225 数据库标识符。使用这个URL连接到the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser?DB=gk_current&ID=10536225 Reactome R-DME-2294590 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser_st_id?ST_ID=R-DME-2294590.1 Phosphorylated threonine T1415 of NCAPD3 condensin II subunit serves as a docking site for PLK1 (Abe et al. 2011). inferred by electronic annotation IEA GO IEA 2.7.11 PLK1 hyperphosphorylates Condensin II complex PLK1 hyperphosphorylates Condensin II complex This event has been computationally inferred from an event that has been demonstrated in another species.

The inference is based on the homology mapping from PANTHER. Briefly, reactions for which all involved PhysicalEntities (in input, output and catalyst) have a mapped orthologue/paralogue (for complexes at least 75% of components must have a mapping) are inferred to the other species. High level events are also inferred for these events to allow for easier navigation.

More details and caveats of the event inference in Reactome. For details on PANTHER see also: http://www.pantherdb.org/about.jsp Reactome DB_ID: 10536206 1 Reactome DB_ID: 29358 1 Reactome DB_ID: 10536218 1 Hyperphosphorylated Condensin II:Nucleosome [nucleoplasm] Hyperphosphorylated Condensin II:Nucleosome Reactome DB_ID: 10536199 1 Reactome DB_ID: 10536216 1 Hyperphosphorylated Condensin II [nucleoplasm] Hyperphosphorylated Condensin II Reactome DB_ID: 10536211 1 Ghost homologue of p-S-NCAPG2 [nucleoplasm] Ghost homologue of p-S-NCAPG2 Reactome DB_ID: 10536214 1 O-phospho-L-serine at unknown position O-phospho-L-serine [MOD:00046] 1 EQUAL 605 EQUAL Reactome DB_ID: 10536209 1 O-phospho-L-threonine at 1415 (in Homo sapiens) 1415 EQUAL O-phospho-L-serine at 1419 (in Homo sapiens) 1419 EQUAL 1 EQUAL 1498 EQUAL Reactome DB_ID: 10535944 1 1 EQUAL 1288 EQUAL Reactome DB_ID: 10535939 1 1 EQUAL 1197 EQUAL Reactome Database ID Release 77 10536216 数据库标识符。使用这个URL连接到the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser?DB=gk_current&ID=10536216 Reactome R-DME-2294598 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser_st_id?ST_ID=R-DME-2294598.1 Reactome DB_ID: 10504772 1 O-phospho-L-threonine at 210 (in Homo sapiens) 210 EQUAL 1 EQUAL 603 EQUAL Reactome Database ID Release 77 10536218 数据库标识符。使用这个URL连接到the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser?DB=gk_current&ID=10536218 Reactome R-DME-2294604 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser_st_id?ST_ID=R-DME-2294604.1 Reactome DB_ID: 113582 1 PHYSIOL-LEFT-TO-RIGHT ACTIVATION Reactome DB_ID: 10536206 GO 0004674 GO molecular function Reactome Database ID Release 77 10536219 数据库标识符。使用这个URL连接到the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser?DB=gk_current&ID=10536219 Reactome Database ID Release 77 10536221 数据库标识符。使用这个URL连接到the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser?DB=gk_current&ID=10536221 Reactome R-DME-2294580 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser_st_id?ST_ID=R-DME-2294580.1 Once PLK1 is recruited to the chromatin-bound condensin II complex, it phosphorylates the NCAPD3 subunit of condensin II on serine residue S1419, and possibly other residues. In addition to phosphorylating NCAPD3, PLK1 phosphorylates other condensin II subunits, NCAPG2 and NCAPH2. However, the phosphorylation sites have not yet been determined. PLK1-mediated phosphorylation of the condensin II complex facilitates condensation of prophase chromosomes (Abe et al. 2011). inferred by electronic annotation IEA GO IEA MCPH1 sequesters condensin II MCPH1 sequesters condensin II This event has been computationally inferred from an event that has been demonstrated in another species.

The inference is based on the homology mapping from PANTHER. Briefly, reactions for which all involved PhysicalEntities (in input, output and catalyst) have a mapped orthologue/paralogue (for complexes at least 75% of components must have a mapping) are inferred to the other species. High level events are also inferred for these events to allow for easier navigation.

More details and caveats of the event inference in Reactome. For details on PANTHER see also: http://www.pantherdb.org/about.jsp Reactome DB_ID: 10536596 1 UniProt:E1JH39 MCPH1 UniProt E1JH39 1 EQUAL 835 EQUAL Reactome DB_ID: 10521442 1 UniProt:P53997 Set UniProt P53997 2 EQUAL 290 EQUAL Reactome DB_ID: 10535958 1 Reactome DB_ID: 10536598 1 Condensin II:MCPH1:SET [nucleoplasm] Condensin II:MCPH1:SET Reactome DB_ID: 10536596 1 1 EQUAL 835 EQUAL Reactome DB_ID: 10521442 1 2 EQUAL 290 EQUAL Reactome DB_ID: 10535958 1 Reactome Database ID Release 77 10536598 数据库标识符。使用这个URL连接到the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser?DB=gk_current&ID=10536598 Reactome R-DME-2429713 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser_st_id?ST_ID=R-DME-2429713.1 Reactome Database ID Release 77 10536600 数据库标识符。使用这个URL连接到the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser?DB=gk_current&ID=10536600 Reactome R-DME-2429719 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser_st_id?ST_ID=R-DME-2429719.1 MCPH1 (microcephalin) binds condensin II complex through direct interaction with NCAPG2 and possibly NCAPD3 condensin II subunits (Wood et al. 2008, Yamashita et al. 2011). MCPH1 binding sequesters condensin II by preventing loading of condensin II on chromatin. Simultaneous binding of MCPH1 to the SET oncogene may contribute to condensin II sequestering (Leung et al. 2011). Mutations in MCPH1 are a cause of microchephaly inhereted in an autosomally recessive manner. MCPH1 deficient cells show premature chromosome condensation (PCC) phenotype, with metaphase-like chromosomes apparent in prophase, before nuclear envelope breakdown (Wood et al. 2008). 21911480 Pubmed 2011 MCPH1 regulates chromosome condensation and shaping as a composite modulator of condensin II Yamashita, Daisuke Shintomi, Keishi Ono, Takao Gavvovidis, Ioannis Schindler, Detlev Neitzel, Heidemarie Trimborn, Marc Hirano, Tatsuya J. Cell Biol. 194:841-54 18718915 Pubmed 2008 Microcephalin/MCPH1 associates with the Condensin II complex to function in homologous recombination repair Wood, Jamie L Liang, Yulong Li, Kaiyi Chen, J J. Biol. Chem. 283:29586-92 21515671 Pubmed 2011 集核与microcephalin致癌基因的同事/MCPH1 and regulates chromosome condensation Leung, Justin W Leitch, Andrea Wood, Jamie L Shaw-Smith, Charles Metcalfe, Kay Bicknell, Louise S Jackson, Andrew P Chen, J J. Biol. Chem. 286:21393-400 inferred by electronic annotation IEA GO IEA Reactome Database ID Release 77 10571181 数据库标识符。使用这个URL连接到the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser?DB=gk_current&ID=10571181 Reactome R-DME-2299718 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser_st_id?ST_ID=R-DME-2299718.1 GO 0007076 GO biological process In mitotic prophase, the action of the condensin II complex enables initial chromosome condensation.

The condensin II complex subunit NCAPD3 binds monomethylated histone H4 (H4K20me1), thereby associating with chromatin (Liu et al. 2010). Binding of the condensin II complex to chromatin is partially controlled by the presence of RB1 (Longworth et al. 2008).

Two mechanisms contribute to the accumulation of H4K20me1 at mitotic entry. First, the activity of SETD8 histone methyltransferase peaks at G2/M transition (Nishioka et al. 2002, Rice et al. 2002, Wu et al. 2010). Second, the complex of CDK1 and cyclin B1 (CDK1:CCNB1) phosphorylates PHF8 histone demethylase at the start of mitosis, removing it from chromatin (Liu et al. 2010).

Condensin II complex needs to be phosphorylated by the CDK1:CCNB1 complex, and then phosphorylated by PLK1, in order to efficiently condense prophase chromosomes (Abe et al. 2011). 20966048 Pubmed 2010 Dynamic regulation of the PR-Set7 histone methyltransferase is required for normal cell cycle progression Wu, Shumin Wang, Weiping Kong, Xiangduo Congdon, Lauren M Yokomori, Kyoko Kirschner, Marc W 大米,居dd C Genes Dev. 24:2531-42 12086618 Pubmed 2002 PR-Set7 is a nucleosome-specific methyltransferase that modifies lysine 20 of histone H4 and is associated with silent chromatin Nishioka, Kenichi 大米,居dd C Sarma, Kavitha Erdjument-Bromage, H Werner, Janis Wang, Yanming Chuikov, Sergei Valenzuela, Pablo Tempst, P Steward, Ruth Lis, John T Allis, C David Reinberg, Danny Mol. Cell 9:1201-13 20622854 Pubmed 2010 PHF8 mediates histone H4 lysine 20 demethylation events involved in cell cycle progression Liu, W Tanasa, B Tyurina, OV Zhou, TY Gassmann, R Liu, WT Ohgi, KA Benner, C Garcia-Bassets, I Aggarwal, AK Desai, A Dorrestein, PC Glass, CK Rosenfeld, MG Nature 466:508-12 12208845 Pubmed 2002 Mitotic-specific methylation of histone H4 Lys 20 follows increased PR-Set7 expression and its localization to mitotic chromosomes 大米,居dd C Nishioka, Kenichi Sarma, Kavitha Steward, Ruth Reinberg, Danny Allis, C David Genes Dev. 16:2225-30 inferred by electronic annotation IEA GO IEA