BioPAX pathway converted from "Platelet sensitization by LDL" in the Reactome database. Platelet sensitization by LDL Platelet sensitization by LDL This event has been computationally inferred from an event that has been demonstrated in another species.

The inference is based on the homology mapping from PANTHER. Briefly, reactions for which all involved PhysicalEntities (in input, output and catalyst) have a mapped orthologue/paralogue (for complexes at least 75% of components must have a mapping) are inferred to the other species. High level events are also inferred for these events to allow for easier navigation.

More details and caveats of the event inference in Reactome. For details on PANTHER see also: http://www.pantherdb.org/about.jsp LDL binds to LRP8 LDL binds to LRP8 This event has been computationally inferred from an event that has been demonstrated in another species.

The inference is based on the homology mapping from PANTHER. Briefly, reactions for which all involved PhysicalEntities (in input, output and catalyst) have a mapped orthologue/paralogue (for complexes at least 75% of components must have a mapping) are inferred to the other species. High level events are also inferred for these events to allow for easier navigation.

More details and caveats of the event inference in Reactome. For details on PANTHER see also: http://www.pantherdb.org/about.jsp Reactome DB_ID: 9966634 1 extracellular region GO 0005576 LDL [extracellular region] LDL Reactome DB_ID: 9966632 1 UniProt:F1P8Z5 APOB Reactome //www.joaskin.com Canis familiaris NCBI Taxonomy 9615 UniProt F1P8Z5 Chain Coordinates 28 EQUAL 4563 EQUAL Reactome DB_ID: 171076 1500 cholesteryl ester [ChEBI:17002] cholesteryl ester ChEBI 17002 Reactome DB_ID: 171126 170 triglyceride [ChEBI:17855] triglyceride ChEBI 17855 Reactome DB_ID: 171149 670 cholesterol [ChEBI:16113] cholesterol cholest-5-en-3beta-ol ChEBI 16113 Reactome DB_ID: 171066 690 phospholipid [ChEBI:16247] phospholipid ChEBI 16247 Reactome Database ID Release 77 9966634 Database identifier. Use this URL to connect to the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser?DB=gk_current&ID=9966634 Reactome R-CFA-171131 1 Reactome稳定的标识符。使用这个URL connect to the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser_st_id?ST_ID=R-CFA-171131.1 Reactome DB_ID: 9984199 1 plasma membrane GO 0005886 UniProt:A0A5F4D4Q6 LRP8 UniProt A0A5F4D4Q6 33 EQUAL 963 EQUAL Reactome DB_ID: 9984201 1 LDL:LRP8 [plasma membrane] LDL:LRP8 Reactome DB_ID: 9966634 1 Reactome DB_ID: 9984199 1 33 EQUAL 963 EQUAL Reactome Database ID Release 77 9984201 Database identifier. Use this URL to connect to the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser?DB=gk_current&ID=9984201 Reactome R-CFA-432119 1 Reactome稳定的标识符。使用这个URL connect to the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser_st_id?ST_ID=R-CFA-432119.1 Reactome Database ID Release 77 9984203 Database identifier. Use this URL to connect to the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser?DB=gk_current&ID=9984203 Reactome R-CFA-432121 1 Reactome稳定的标识符。使用这个URL connect to the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser_st_id?ST_ID=R-CFA-432121.1 LPR8 (apoER2) is the platelet low density lipoprotein (LDL) receptor. Mice lacking ApoE develop hypercholesterolemia and later atherosclerosis (Zhang et al. 1992). Similiar results are seen in familial hypercholesterolemia, where defective apoB/E receptors fail to remove LDL from the circulation. 1411543 Pubmed 1992 Spontaneous hypercholesterolemia and arterial lesions in mice lacking apolipoprotein E Zhang, SH Reddick, RL Piedrahita, JA Maeda, N Science 258:468-71 15459198 Pubmed 2004 Binding of low density lipoprotein to platelet apolipoprotein E receptor 2' results in phosphorylation of p38MAPK Korporaal, SJ Relou, IA van Eck, M Strasser, V Bezemer, M Gorter, G van Berkel, TJ Nimpf, J Akkerman, Jan Willem N Lenting, PJ J Biol Chem 279:52526-34 inferred by electronic annotation IEA GO IEA FGR binds LDL:LRP8 FGR binds LDL:LRP8 This event has been computationally inferred from an event that has been demonstrated in another species.

The inference is based on the homology mapping from PANTHER. Briefly, reactions for which all involved PhysicalEntities (in input, output and catalyst) have a mapped orthologue/paralogue (for complexes at least 75% of components must have a mapping) are inferred to the other species. High level events are also inferred for these events to allow for easier navigation.

More details and caveats of the event inference in Reactome. For details on PANTHER see also: http://www.pantherdb.org/about.jsp Reactome DB_ID: 9984201 1 Reactome DB_ID: 9984207 1 cytosol GO 0005829 UniProt:E2RSN0 FGR UniProt E2RSN0 1 EQUAL 529 EQUAL Reactome DB_ID: 9984209 1 LDL:LRP8:FGR [plasma membrane] LDL:LRP8:FGR Reactome DB_ID: 9984201 1 Reactome DB_ID: 9984207 1 1 EQUAL 529 EQUAL Reactome Database ID Release 77 9984209 Database identifier. Use this URL to connect to the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser?DB=gk_current&ID=9984209 Reactome R-CFA-8948036 1 Reactome稳定的标识符。使用这个URL connect to the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser_st_id?ST_ID=R-CFA-8948036.1 Reactome Database ID Release 77 10036671 Database identifier. Use this URL to connect to the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser?DB=gk_current&ID=10036671 Reactome R-CFA-8948034 1 Reactome稳定的标识符。使用这个URL connect to the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser_st_id?ST_ID=R-CFA-8948034.1 Tyrosine phosphorylation of LDL:LRP8 is mediated by the Src-family kinase FGR, based on a correlation of increased LRP8 phosphorylation in LDL-stimulated platelets and a transient increased co-precipitation of FGR and LRP8 upon LDL stimulation. inferred by electronic annotation IEA GO IEA 2.7.10 FGR binds and phosphorylates LRP8 FGR binds and phosphorylates LRP8 This event has been computationally inferred from an event that has been demonstrated in another species.

The inference is based on the homology mapping from PANTHER. Briefly, reactions for which all involved PhysicalEntities (in input, output and catalyst) have a mapped orthologue/paralogue (for complexes at least 75% of components must have a mapping) are inferred to the other species. High level events are also inferred for these events to allow for easier navigation.

More details and caveats of the event inference in Reactome. For details on PANTHER see also: http://www.pantherdb.org/about.jsp Reactome DB_ID: 9984209 1 Reactome DB_ID: 113592 1 ATP(4-) [ChEBI:30616] ATP(4-) Adenosine 5'-triphosphate atp ATP ChEBI 30616 Reactome DB_ID: 29370 1 ADP(3-) [ChEBI:456216] ADP(3-) ADP trianion 5'-O-[(phosphonatooxy)phosphinato]adenosine ADP ChEBI 456216 Reactome DB_ID: 9984216 1 LDL:p-LRP8:FGR [plasma membrane] LDL:p-LRP8:FGR Reactome DB_ID: 9984214 1 LDL:p-LRP8 [plasma membrane] LDL:p-LRP8 Reactome DB_ID: 9966634 1 Reactome DB_ID: 9984212 1 O4的-phospho-L-tyrosine at unknown position O4的-phospho-L-tyrosine [MOD:00048] 33 EQUAL 963 EQUAL Reactome Database ID Release 77 9984214 Database identifier. Use this URL to connect to the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser?DB=gk_current&ID=9984214 Reactome R-CFA-432133 1 Reactome稳定的标识符。使用这个URL connect to the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser_st_id?ST_ID=R-CFA-432133.1 Reactome DB_ID: 9984207 1 1 EQUAL 529 EQUAL Reactome Database ID Release 77 9984216 Database identifier. Use this URL to connect to the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser?DB=gk_current&ID=9984216 Reactome R-CFA-432132 1 Reactome稳定的标识符。使用这个URL connect to the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser_st_id?ST_ID=R-CFA-432132.1 PHYSIOL-LEFT-TO-RIGHT ACTIVATION Reactome DB_ID: 9984209 GO 0004713 GO molecular function Reactome Database ID Release 77 9984217 Database identifier. Use this URL to connect to the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser?DB=gk_current&ID=9984217 Reactome Database ID Release 77 9984219 Database identifier. Use this URL to connect to the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser?DB=gk_current&ID=9984219 Reactome R-CFA-432129 1 Reactome稳定的标识符。使用这个URL connect to the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser_st_id?ST_ID=R-CFA-432129.1 Tyrosine phosphorylation of LDL:LRP8 is mediated by the Src-family kinase FGR, based on a correlation of increased LRP8 phosphorylation on LDL stimulation of platelets, and a transient increased co-precipitation of FGR with LRP8 upon LDL stimulation. inferred by electronic annotation IEA GO IEA 2.7.10.2 Fgr may phosphorylate p38 MAPK Fgr may phosphorylate p38 MAPK This event has been computationally inferred from an event that has been demonstrated in another species.

The inference is based on the homology mapping from PANTHER. Briefly, reactions for which all involved PhysicalEntities (in input, output and catalyst) have a mapped orthologue/paralogue (for complexes at least 75% of components must have a mapping) are inferred to the other species. High level events are also inferred for these events to allow for easier navigation.

More details and caveats of the event inference in Reactome. For details on PANTHER see also: http://www.pantherdb.org/about.jsp Reactome DB_ID: 9983656 1 UniProt:O02812 MAPK14 UniProt O02812 2 EQUAL 360 EQUAL Reactome DB_ID: 113592 2 Reactome DB_ID: 9968478 1 O-phospho-L-threonine at 180 (in Homo sapiens) 180 EQUAL O-phospho-L-threonine [MOD:00047] O4的-phospho-L-tyrosine at 182 (in Homo sapiens) 182 EQUAL 2 EQUAL 360 EQUAL Reactome DB_ID: 29370 2 PHYSIOL-LEFT-TO-RIGHT ACTIVATION Reactome DB_ID: 9984207 1 EQUAL 529 EQUAL GO 0004715 GO molecular function Reactome Database ID Release 77 9984224 Database identifier. Use this URL to connect to the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser?DB=gk_current&ID=9984224 Reactome Database ID Release 77 9984226 Database identifier. Use this URL to connect to the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser?DB=gk_current&ID=9984226 Reactome R-CFA-432148 1 Reactome稳定的标识符。使用这个URL connect to the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser_st_id?ST_ID=R-CFA-432148.1 LDL stimulation of platelets leads to increased p38 MAPK activation by phosphorylation. An Src family kinase is responsible for this; Fgr is a strong candidate as it is known to bind the LDL receptor in platelets responding to LDL and in chemoattractant-induced degranulation of neutrophils activation of p38 MAPK is blocked by a triple Hck/Fgr/Lyn knockout. However fMLP-stimulated phosphorylation of MAPKs in a double hck/fgr PMNs was observed to be normal, suggesting that Lyn, rather than Fyn, is involved. 17339487 Pubmed 2007 The Src family kinases Hck and Fgr regulate neutrophil responses to N-formyl-methionyl-leucyl-phenylalanine Fumagalli, L Zhang, H Baruzzi, A Lowell, CA Berton, G J Immunol 178:3874-85 10754332 Pubmed 2000 Kinase pathways in chemoattractant-induced degranulation of neutrophils: the role of p38 mitogen-activated protein kinase activated by Src family kinases Mocsai, A Jakus, Z Vántus, T Berton, G Lowell, CA Ligeti, E J Immunol 164:4321-31 inferred by electronic annotation IEA GO IEA 2.7.11 Phosphorylation of cPLA2 by MAPK p38 alpha Phosphorylation of cPLA2 by MAPK p38 alpha This event has been computationally inferred from an event that has been demonstrated in another species.

The inference is based on the homology mapping from PANTHER. Briefly, reactions for which all involved PhysicalEntities (in input, output and catalyst) have a mapped orthologue/paralogue (for complexes at least 75% of components must have a mapping) are inferred to the other species. High level events are also inferred for these events to allow for easier navigation.

More details and caveats of the event inference in Reactome. For details on PANTHER see also: http://www.pantherdb.org/about.jsp Reactome DB_ID: 9983660 1 UniProt:F6UQ33 PLA2G4A UniProt F6UQ33 1 EQUAL 749 EQUAL Reactome DB_ID: 113592 2 Reactome DB_ID: 9961902 1 endoplasmic reticulum membrane GO 0005789 O-phospho-L-serine at 505 (in Homo sapiens) 505 EQUAL O-phospho-L-serine [MOD:00046] O-phospho-L-serine at 727 (in Homo sapiens) 727 EQUAL 1 EQUAL 749 EQUAL Reactome DB_ID: 29370 2 PHYSIOL-LEFT-TO-RIGHT ACTIVATION Reactome DB_ID: 9968478 O-phospho-L-threonine at 180 (in Homo sapiens) 180 EQUAL O4的-phospho-L-tyrosine at 182 (in Homo sapiens) 182 EQUAL 2 EQUAL 360 EQUAL GO 0004674 GO molecular function Reactome Database ID Release 77 9983661 Database identifier. Use this URL to connect to the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser?DB=gk_current&ID=9983661 Reactome Database ID Release 77 9983663 Database identifier. Use this URL to connect to the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser?DB=gk_current&ID=9983663 Reactome R-CFA-428961 1 Reactome稳定的标识符。使用这个URL connect to the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser_st_id?ST_ID=R-CFA-428961.1 MAPK p38 alpha activates cPLA2 by phosphorylation of two serine residues.
cPLA2 can be phosphorylated and activated by ERK2 (Lin et al. 1993), and were believed to be responsible for the phosphorylation of cPLA2. However, phosphorylation of cPLA2 occurred in the absence of ERK activation in human platelets stimulated with the thrombin receptor agonist peptide SFLLRN (Kramer et al. 1995), and cPLA2 phosphorylation induced by thrombin or collagen was unaffected by PKC inhibitors that prevent ERK activation (Börsch-Haubold et al. 1995). In addition, a specific inhibitor of ERKs did not block thrombin-induced cPLA2 phosphorylation (Börsch-Haubold et al. 1996). 9468497 Pubmed 1998 cyt鉴定磷酸化位点osolic phospholipase A2 in agonist-stimulated human platelets and HeLa cells Börsch-Haubold, AG Bartoli, F Asselin, J Dudler, T Kramer, RM Apitz-Castro, R Watson, SP Gelb, Michael H J Biol Chem 273:4449-58 8910365 Pubmed 1996 p38增殖蛋白激酶磷酸化s cytosolic phospholipase A2 (cPLA2) in thrombin-stimulated platelets. Evidence that proline-directed phosphorylation is not required for mobilization of arachidonic acid by cPLA2 Kramer, RM Roberts, EF Um, SL Börsch-Haubold, AG Watson, SP Fisher, MJ Jakubowski, JA J Biol Chem 271:27723-9 7782348 Pubmed 1995 Differential activation of cytosolic phospholipase A2 (cPLA2) by thrombin and thrombin receptor agonist peptide in human platelets. Evidence for activation of cPLA2 independent of the mitogen-activated protein kinases ERK1/2 Kramer, RM Roberts, EF Hyslop, PA Utterback, BG Hui, KY Jakubowski, JA J Biol Chem 270:14816-23 8761473 Pubmed 1996 Inhibition of mitogen-activated protein kinase kinase does not impair primary activation of human platelets Börsch-Haubold, AG Kramer, RM Watson, SP Biochem J 318:207-12 8381049 Pubmed 1993 cPLA2 is phosphorylated and activated by MAP kinase Lin, LL Wartmann, M Lin, AY Knopf, JL Seth, A Davis, RJ Cell 72:269-78 7592775 Pubmed 1995 Cytosolic phospholipase A2 is phosphorylated in collagen- and thrombin-stimulated human platelets independent of protein kinase C and mitogen-activated protein kinase Börsch-Haubold, AG Kramer, RM Watson, SP J Biol Chem 270:25885-92 inferred by electronic annotation IEA GO IEA PECAM1 is phosphorylated PECAM1 is phosphorylated This event has been computationally inferred from an event that has been demonstrated in another species.

The inference is based on the homology mapping from PANTHER. Briefly, reactions for which all involved PhysicalEntities (in input, output and catalyst) have a mapped orthologue/paralogue (for complexes at least 75% of components must have a mapping) are inferred to the other species. High level events are also inferred for these events to allow for easier navigation.

More details and caveats of the event inference in Reactome. For details on PANTHER see also: http://www.pantherdb.org/about.jsp Reactome DB_ID: 9972505 1 UniProt:F1PYL8 PECAM1 UniProt F1PYL8 28 EQUAL 738 EQUAL Reactome DB_ID: 113592 2 Reactome DB_ID: 29370 2 Reactome DB_ID: 9974000 1 O4的-phospho-L-tyrosine at 663 (in Homo sapiens) 663 EQUAL O4的-phospho-L-tyrosine at 686 (in Homo sapiens) 686 EQUAL 27 EQUAL -1 EQUAL Reactome Database ID Release 77 9984583 Database identifier. Use this URL to connect to the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser?DB=gk_current&ID=9984583 Reactome R-CFA-435244 1 Reactome稳定的标识符。使用这个URL connect to the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser_st_id?ST_ID=R-CFA-435244.1 LDL causes a transient increase in p38 MAPK activity in platelets. After an initial phase in which LDL leads to the activation of p38MAPK, LDL leads to tyrosine phosphorylation and thereby activation of PECAM-1, which in turn leads to binding and stimulation of the Ser/Thr phosphatases PP1/PP2A which reduce the activity of p38MAPK by dephosphorylation. 12689916 Pubmed 2003 Signal transduction pathways mediated by PECAM-1: new roles for an old molecule in platelet and vascular cell biology Newman, Peter J Newman, Debra K Arterioscler. Thromb. Vasc. Biol. 23:953-64 12775720 Pubmed 2003 Platelet endothelial cell adhesion molecule-1 (PECAM-1) inhibits low density lipoprotein-induced signaling in platelets Relou, IA Gorter, G Ferreira, IA van Rijn, HJ Akkerman, Jan Willem N J Biol Chem 278:32638-44 inferred by electronic annotation IEA GO IEA ACTIVATION Reactome Database ID Release 77 9984584 Database identifier. Use this URL to connect to the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser?DB=gk_current&ID=9984584 Reactome DB_ID: 9966634 PECAM-1 binds PP2A PECAM-1 binds PP2A This event has been computationally inferred from an event that has been demonstrated in another species.

The inference is based on the homology mapping from PANTHER. Briefly, reactions for which all involved PhysicalEntities (in input, output and catalyst) have a mapped orthologue/paralogue (for complexes at least 75% of components must have a mapping) are inferred to the other species. High level events are also inferred for these events to allow for easier navigation.

More details and caveats of the event inference in Reactome. For details on PANTHER see also: http://www.pantherdb.org/about.jsp Reactome DB_ID: 9963398 1 PP2A [cytosol] PP2A Converted from EntitySet in Reactome Reactome DB_ID: 9963390 1 PP2A-subunit A [cytosol] Converted from EntitySet in Reactome. Each synonym is a name of a PhysicalEntity, and each XREF points to one PhysicalEntity Converted from EntitySet in Reactome Reactome DB_ID: 9963396 1 PP2A-catalytic亚基C(胞质) Converted from EntitySet in Reactome. Each synonym is a name of a PhysicalEntity, and each XREF points to one PhysicalEntity Converted from EntitySet in Reactome Reactome DB_ID: 9963384 1 PP2A regulatory subunit B56 [cytosol] Converted from EntitySet in Reactome. Each synonym is a name of a PhysicalEntity, and each XREF points to one PhysicalEntity Reactome Database ID Release 77 9963398 Database identifier. Use this URL to connect to the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser?DB=gk_current&ID=9963398 Reactome R-CFA-196206 1 Reactome稳定的标识符。使用这个URL connect to the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser_st_id?ST_ID=R-CFA-196206.1 Reactome DB_ID: 9974000 1 O4的-phospho-L-tyrosine at 663 (in Homo sapiens) 663 EQUAL O4的-phospho-L-tyrosine at 686 (in Homo sapiens) 686 EQUAL 27 EQUAL -1 EQUAL Reactome DB_ID: 9984221 1 p-PECAM:PP2A [plasma membrane] p-PECAM:PP2A Reactome DB_ID: 9963398 1 Reactome DB_ID: 9974000 1 O4的-phospho-L-tyrosine at 663 (in Homo sapiens) 663 EQUAL O4的-phospho-L-tyrosine at 686 (in Homo sapiens) 686 EQUAL 27 EQUAL -1 EQUAL Reactome Database ID Release 77 9984221 Database identifier. Use this URL to connect to the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser?DB=gk_current&ID=9984221 Reactome R-CFA-432144 1 Reactome稳定的标识符。使用这个URL connect to the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser_st_id?ST_ID=R-CFA-432144.1 Reactome Database ID Release 77 9984223 Database identifier. Use this URL to connect to the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser?DB=gk_current&ID=9984223 Reactome R-CFA-432143 1 Reactome稳定的标识符。使用这个URL connect to the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser_st_id?ST_ID=R-CFA-432143.1 PECAM-1 co-immunoprecipitates with PP2A inferred by electronic annotation IEA GO IEA Interaction of PECAM-1 and SHP-1 Interaction of PECAM-1 and SHP-1 This event has been computationally inferred from an event that has been demonstrated in another species.

The inference is based on the homology mapping from PANTHER. Briefly, reactions for which all involved PhysicalEntities (in input, output and catalyst) have a mapped orthologue/paralogue (for complexes at least 75% of components must have a mapping) are inferred to the other species. High level events are also inferred for these events to allow for easier navigation.

More details and caveats of the event inference in Reactome. For details on PANTHER see also: http://www.pantherdb.org/about.jsp Reactome DB_ID: 9973770 1 UniProt:A0A5F4BS19 PTPN6 UniProt A0A5F4BS19 1 EQUAL 595 EQUAL Reactome DB_ID: 9974000 1 O4的-phospho-L-tyrosine at 663 (in Homo sapiens) 663 EQUAL O4的-phospho-L-tyrosine at 686 (in Homo sapiens) 686 EQUAL 27 EQUAL -1 EQUAL Reactome DB_ID: 9974002 1 PECAM-1:SHP-1 complex [plasma membrane] PECAM-1:SHP-1 complex Reactome DB_ID: 9973770 1 1 EQUAL 595 EQUAL Reactome DB_ID: 9974000 1 O4的-phospho-L-tyrosine at 663 (in Homo sapiens) 663 EQUAL O4的-phospho-L-tyrosine at 686 (in Homo sapiens) 686 EQUAL 27 EQUAL -1 EQUAL Reactome Database ID Release 77 9974002 Database identifier. Use this URL to connect to the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser?DB=gk_current&ID=9974002 Reactome R-CFA-210221 1 Reactome稳定的标识符。使用这个URL connect to the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser_st_id?ST_ID=R-CFA-210221.1 Reactome Database ID Release 77 9974004 Database identifier. Use this URL to connect to the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser?DB=gk_current&ID=9974004 Reactome R-CFA-210277 1 Reactome稳定的标识符。使用这个URL connect to the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser_st_id?ST_ID=R-CFA-210277.1 The phosphorylation of two tandem tyrosine residues (Y663 and Y686) within the cytoplasmic domain of PECAM-1 is required for the downstream signalling events observed following PECAM-1 ligation. Both SH2 domains of SHP-1 are required in tandem to bind PECAM-1. 9774457 Pubmed 1998 Recruitment and activation of SHP-1 protein-tyrosine phosphatase by human platelet endothelial cell adhesion molecule-1 (PECAM-1). Identification of immunoreceptor tyrosine-based inhibitory motif-like binding motifs and substrates. Hua, CT Gamble, JR Vadas, MA Jackson, DE J Biol Chem 273:28332-40 10350061 Pubmed 1999 Differential association of cytoplasmic signalling molecules SHP-1, SHP-2, SHIP and phospholipase C-gamma1 with PECAM-1/CD31 Pumphrey, NJ Taylor, V Freeman, S Douglas, MR Bradfield, PF Young, SP Lord, JM Wakelam, MJ Bird, IN Salmon, M Buckley, CD FEBS Lett 450:77-83 inferred by electronic annotation IEA GO IEA Interaction of PECAM-1 and SHP-2 Interaction of PECAM-1 and SHP-2 This event has been computationally inferred from an event that has been demonstrated in another species.

The inference is based on the homology mapping from PANTHER. Briefly, reactions for which all involved PhysicalEntities (in input, output and catalyst) have a mapped orthologue/paralogue (for complexes at least 75% of components must have a mapping) are inferred to the other species. High level events are also inferred for these events to allow for easier navigation.

More details and caveats of the event inference in Reactome. For details on PANTHER see also: http://www.pantherdb.org/about.jsp Reactome DB_ID: 9967884 1 UniProt:F1Q3J6 PTPN11 UniProt F1Q3J6 1 EQUAL 597 EQUAL Reactome DB_ID: 9974000 1 O4的-phospho-L-tyrosine at 663 (in Homo sapiens) 663 EQUAL O4的-phospho-L-tyrosine at 686 (in Homo sapiens) 686 EQUAL 27 EQUAL -1 EQUAL Reactome DB_ID: 9974037 1 PECAM-1:SHP-2 complex [plasma membrane] PECAM-1:SHP-2 complex Reactome DB_ID: 9967884 1 1 EQUAL 597 EQUAL Reactome DB_ID: 9974000 1 O4的-phospho-L-tyrosine at 663 (in Homo sapiens) 663 EQUAL O4的-phospho-L-tyrosine at 686 (in Homo sapiens) 686 EQUAL 27 EQUAL -1 EQUAL Reactome Database ID Release 77 9974037 Database identifier. Use this URL to connect to the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser?DB=gk_current&ID=9974037 Reactome R-CFA-210219 1 Reactome稳定的标识符。使用这个URL connect to the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser_st_id?ST_ID=R-CFA-210219.1 Reactome Database ID Release 77 9974039 Database identifier. Use this URL to connect to the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser?DB=gk_current&ID=9974039 Reactome R-CFA-210294 1 Reactome稳定的标识符。使用这个URL connect to the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser_st_id?ST_ID=R-CFA-210294.1 PECAM-1 becomes tyrosine-phosphorylated during the platelet aggregation process; the phosphorylation of two tandem tyrosine residues (Y663 and Y686) within the cytoplasmic domain is required for downstream signalling events. Phosphorylation creates docking sites for the protein-tyrosine phosphatase SHP-2. The interaction between SHP-2 and PECAM-1 is dependent upon integrin-mediated platelet/platelet interactions and occurs via the Src homology 2 (SH2) domains of the phosphatase and highly conserved phosphatase-binding motifs encompassing phosphotyrosines 663 and 686 within the cytoplasmic domain of PECAM-1. 9054388 Pubmed 1997 The protein-tyrosine phosphatase SHP-2 binds platelet/endothelial cell adhesion molecule-1 (PECAM-1) and forms a distinct signaling complex during platelet aggregation. Evidence for a mechanistic link between PECAM-1- and integrin-mediated cellular signaling. Jackson, DE Ward, CM Wang, R Newman, PJ J Biol Chem 272:6986-93 inferred by electronic annotation IEA GO IEA Reactome Database ID Release 77 10045245 Database identifier. Use this URL to connect to the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser?DB=gk_current&ID=10045245 Reactome R-CFA-432142 1 Reactome稳定的标识符。使用这个URL connect to the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser_st_id?ST_ID=R-CFA-432142.1 Physiological concentrations (1g/L) of Low density lipoprotein (LDL) enhance platelet aggregation responses initiated by thrombin, collagen, and ADP. This enhancement involves the rapid phosphorylation of p38 mitogen-activated protein kinase (p38MAPK) at Thr180 and Tyr182. The receptor for LDL is ApoER2, a splice variant of the classical ApoE receptor. ApoER2 stimulation leads to association of the Src family kinase Fgr which is probably responsible for subsequent phosphorylation of p38MAPK. This stimulation is transient because LDL also increases the activity of PECAM-1, which stimulates phosphatases that dephosphorylate p38MAPK. 12038793 Pubmed 2002 Platelet activation by the apoB/E receptor-binding domain of LDL Relou, AM Gorter, G van Rijn, HJ Akkerman, Jan Willem N Thromb Haemost 87:880-7 inferred by electronic annotation IEA GO IEA